Equipment Laboratory Services
BIRAD facilitates the establishment of scientific service contracts, in which members of the industrial community can take advantage of the Universitys outstanding knowledge base and technical infrastructure. By sparing businesses the expense of acquiring certain specialized equipment, techniques, etc., BIRADs scientific service division provides cost-effective outsourced services to benefit the business sector.
Along with access to state-of-the-art facilities for life science, chemistry nanotechnology and other fields of research, service contracts provide clients with expert guidance on client-initiated studies from PhD-level technicians, who assist in experiment design and execution, as well as data analysis.
BIRADs scientific service division assists Industrial partners to find the right scientific service and contracts
Biomedical industries that require top-level instrumentation for their research and development turn to Bar-Ilan for their advanced Life Science infrastructure. Highly-trained technicians – most of them graduates of post-doctoral fellowships at distinguished research institutions – supervise experimentation and help clients make use of the following technologies:
- Light Microscopy (fluorescent, confocal, and live-imaging workstations)
- High-Throughput Sequencing – Ion Torrent
- QX100 Droplet Digital PCR System from Bio-Rad
- Biomolecular Interaction Analysis
- HPLC Services
- BioTek Synergy4 Multimode Plate Reader
- FACSAriaIII Cell Sorter with Five Lasers
- In-Vivo Imaging Unit
- Histology Unit
|ECL and BAS-1500|
|FACS ARIA III FACSVantage sorter
Flow Cytometry is the measurement of single cells as they flow one by one past laser beams with the resulting light scatter and fluorescence emission detected and analyzed. Multiple biophysical properties of each individual cell can be measured at rates of thousands of cells per second. Cells are identified by their staining with fluorescent dyes that bind directly to specific constituents like DNA, RNA, proteins or ions or with dyes conjugated to molecules like antibodies, lectins, or hormones that bind to different cellular structures. Cells may also be identified following an infection or tranfection by marker fluorescent proteins. Flow Cytometry can detect and analyze multiple fluorescent staining simultaneously in each individual cell and can report and physically separate them by these qualities.
|FACS Calibur analyzer|
|Full Service Biacore™ T100 Analysis Includes sample preparation, expert advisory services, literature search|
|Biotek Synergy4 Multidetection Plate Reader
Synergy4 is an advanced plate reader with multiple read modes. Detection modes include UV-visible absorbance, fluorescence, time-resolved fluorescence, and luminescence modes. The wavelength selection for absorbance mode is monochromator-based, while in fluorescence mode either monochromator or light-blocking filters and dichroic mirrors for increased sensitivity are used in the optical path. The reader includes dual injector system for in-situ addition of reagents for walk-away convenience and to achieve well synchronization.
|HPLC- purification (Detection Mode: LC-DAD, up to 10mL/min scale)|
|HPLC- Basic HPLC analysis (Detection modes: FL and LC-DAD)
Hitachi LaChrom Elite is a high-performance chromatography system equipped with sophisticated photometric detectors. It is used mainly as an analytical instrument for reverse-phase liquid chromatography. In this capacity, it can be used to perform complex analysis of biological extracts, polypeptides, oligosaccharides, and nucleic acids.
|Maestro In-Vivo Imaging
CRi Maestro II ™ In-Vivo Imaging System is used to image fluorescent-lables in whole-live animals. Using multispectral acquisition and analysis, the system eliminates autofluorescence and provides multicolor flexibility and quantitative accuracy for both visible and near-infrared labels.
The QX100 Droplet Digital PCR (ddPCR) system provides an absolute measure of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The Droplet Digital PCR system is the third generation of PCR technology and provides a revolutionary approach to target DNA quantification.
|Feature extraction with SpotReader|
|Ion Torrent services,
Deep sequencing, also known as high-throughput sequencing, or next generation sequencing is a relatively new technology based on massively parallel sequencing of millions of different DNA fragments, therefore resulting in ultra-high-throughput data.
We offer full gene expression services. We provide consulting, training and analysis services for researchers using one-color or two-color mode of microarrays. The services include consultation on experimental design, choice of appropriate microarray platform, selection of analysis methods or tools, and annotation of microarrays.
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of RNA and DNA. This device can be used to check the quality of RNA samples (namely RNA integrity) prior to microarray processing, real-time PCR and RNA-seq to ensure high-quality RNA. DNA chips are used to analyze libraries before loading them on the Ion Torrent.
|Full service for Imaging|
Research microscope OLYMPUS SZX12 offers long working distance, wide zoom range, excellent image clarity and easy operation to allow a wide range of application.
|Live Imaging – Olympus
Inverted imaging system applicable for Phase-contrast (Ph) and Fluorescence (FL) observations. This microscope is fully motorized and controlled by Olympus software.
|Live Imaging – Zeiss
Inverted imaging system applicable for Brightfield (BF), Phase-contrast (Ph), DIC and Fluorescence (FL) observations. This microscope is fully motorized and controlled by Zeiss ZEN software.
|Confocal Olympus FV1000
The Olympus FluoViewTM FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 an ideal choice for FRAP, FLIP and photoactivation. It is hooked to fully motorized Olympus IX81 inverted microscope.
|Confocal Zeiss-LSM 510 META
LSM 510 META laser scanning confocal microscope from Zeiss is a spectral imaging confocal system. It is hooked with inverted fully motorized Axiovert 200M Zeiss microscope
|Confocal Leica SPE,
The Leica SPE confocal is a true point-scanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells.
The Zeiss AxioImager Z1 is upright, fully motorized, PC controlled high-performance research microscope capable of Brightfield, DIC (Diffferential Interference Contrast) and Fluorescent imaging. The system has motorized Z drive and can be used to acquire 3D images. The microscope has an Axiocam HRm and colour AxioCam MRc cameras. Zeiss AxioVision software controls the microscope and allows multi-dimensional data acquisition (multiple fluorescence channels, Z-stacks, time lapse)
Research system microscope AX70 with Microscope controller Olympus U –MCB effective for Brightfield (BF) , Fluorescence (FL) and Multi (FL+BF) observation. The U-MCB operate via the touch panel while setting the photomicrography conditions and various motorized units.
Bar-Ilans Institute of Nanotechnology and Advanced Materials (BINA) is home to a rich instrumentation infrastructure, which is made available to collaborators from private industry. BINAs five service units – staffed by PhD-level technicians – offer outside users outstanding professional services, and complete support in the following areas:
- Electron microscopy
- Surface analysis
- Magnetic measurement
|e-Beam Lithography, CRESTEC, CABL-9500C
· Acceleration voltage 50KeV
· Minimum beam diameter -2nm
· Minimum line width -8nm
· Stitching accuracy <10nm · 4 wafer sample holder
|SEM – scanning electron microscope, FEI, Inspect
· Low/high vacuum
· Detectors: SE. BSED, EDS, mapping and line scanning
· Resolution 3.3nm
|ESEM – environmental scanning electron microscope, FEI, Quanta
· Filed emission gun (schottky filed emitter)
· High/low vacuum
· High vacuum with beam deceleration
· Extended vacuum mode (wet-ESEM)
· High and low temperature imaging in the range of -20c-+1000c
· Detectors: SE, BSED, vCD, EDS, STEM
· Resolution 1.2nm
|TEM – transmission electron microscope, FEI, tecnai G2, high contrast / cryo TEM
· Accelerating voltage120kV
· Bottom CCD camera 1Kx1K
· Top CCD camera 1Kx1K
· Cryo TEM- direct imaging at cryogenic temperatures
· Resolution 0.46 nm (Lattice resolution)
|TEM – transmission electron microscope, JEOL 1400, Analytical TEM
· Accelerating voltage 120kV
· Bottom CCD 2x2k camera
· Specimen tilt +/- 70c
· Detectors: BF/DF, STEM, EDS, (UltraDry SDD)
· STEM image resolution 2.0 nm
· Resolution 0.38 nm (point to point), 0.20 nm (lattice)
|XHR-SEM – High resolution scanning electron microscope, FEI, Magellan 400L
· Filed emission gun (schttoky field emitter UC (UniColore))
· High vacuum
· Detectors: TLD, Vcd, SE, EDS, EBSD, STEM
· Resolution 0.6nm
|XHR-TEM – High resolution transmission electron microscope, JEOL, JEM-2100
· Accelerating voltage 200kV
· Gatan USC 4000 4x4k camera
· Specimen tilt +/-70c
· Double tilt (+/-45c) vacuum transfer holder
· Detectors: BF/DF STEM, EDS 3D Tomography
· Resolution 0.23 nm (point to point), 0.14 nm (lattice)
|FIB – Focused Ion Beam, FEI, Helios NanoLab
· Dual beam FIB
· Micro probing
· Cross section
· TEM sample preparation
|Evaporation Sputtering, Bestec, GMBH
· Sputtering chamber 2 DC sources, 1 RF source and Ion beam source for milling
· Evaporation chamber with 2 thermal source (high and low temp) and 1 e-beam source with 4 crucibles and a thickness monitor
· Sample temperature control up to 8000C
· Joined load lock chamber with sample caste for 5-sample holder up to 4sample/wafer
· 4 wafer sample holder
|ALD – Atomic layer deposition, Cambridge, Fiji F200
· 4 precursors lines with heated sources
· 4 gas inlets
· Turbo pump with APC mechanism for high aspect ratio samples
· Load lock chamber
· 8 sample holder
|PLD – Pulse Laser Deposition, Neocera, pioneer 180 CCS
· 248nm KrF2 excimer laser
· up to 400 mJ pulse energy
· 4 sample holder, heatable up to 850c
· Deposition from up to 6 targets
· Deposition of homogeneous thickness
· Deposition of compositional gradients
|ICP/RIE – Reactive Ion etcher, Plasma-Therm, VERSALINE
· Semi-Automatic Load Lock for single wafer
· ICP-ll source with 2.5KW/2MHz RF source
· Low temperature heat exchanger down to -40C
· 8 gas lines
· OES endpoint for process control
Bar-Ilan Universitys advanced infrastructure for research in chemistry is available for use by industrial and academic partners, who design, execute and analyze their experiments with the help of the Universitys PhD-level technicians. Specialized instrumentation facilities include:
- X-Ray Diffraction (XRD)
- X-Ray Photoelectron Spectroscopy (XPS)
- Electron Spin Resonance (ESR)
- Molecular Modeling for Nanomaterials
|Circular dichroism spectroscopy (CD)
Circular dichroism spectroscopy is a useful technique for analyzing peptide and protein secondary structure and folding properties in solution using very small amounts of protein or peptides. The new Chirascan CD spectrometer (Applied Photophysics) is available for all faculty members and external users.
· CD measurement
|Molecular modeling and computer-driven materials design
Using molecular modeling computer software has a proven potential for streamlining and optimizing development processes such as pharmaceuticals and nanomaterials. The Department of Chemistry owns an advanced computational platform, both on the hardware and software level, enabling a wide variety of structural and energetic calculations (quantum computations, minimization of energy, conformational searches, molecular dynamics, anchoring, statistical modeling and more) on systems ranging from the atomic level to the crystal lattice cell unit. The calculations are carried out by several research groups with many years of experience in computational chemistry, both in academia and industry.
|Elemental Analysis (CHNS , O)
The centre for Chemical Services is equipped with a Thermo CHNS-O elemental analysis device, model EA 1110, capable of detection of the elements C,H,N,S and O.
· Elemental Analysis
Surface area measurements and adsorption isotherm can be performed for solids and powders using a Quantachrome NOVA 3200E device.
· BET measurement
|Electron Spin Resonance (ESR)
The Unit for EPR (or ESR) owns modern equipment that provides the necessary resources for contemporary research in organo-metallic chemistry, organic radicals, radicals in biology research, antioxidants, electrochemistry, radicals in nano-chemistry and protein research. The unit holds an advanced Bruker, ElexSys 500 instrument (4°- 300 ° K) and an EPR spectrometer – the Bruker EMX 100d.
· EPR measurement
· Spectral analysis, signal simulation and consonance
|Inductively Coupled Plasma (ICP)
Different elements, including halogens can be detected using the ICP technique, with a Horiba JOBIN-YVON ULTIMA2 device.
· 1-3 elements per sample
· 4-6 elements per sample
· 6-8 elements per sample
· More than 8 elements per sample
|X-Ray Photoelectron Spectroscopy (XPS)
The XPS method allows study of the chemical composition of the surface of materials. Information obtained by this method includes the identification of different elements (except hydrogen) and relative quantity. The Centre holds a Kratos Analytical XPS instrument, model AXIS-HS.
· Per measurement
· Spectral analysis and consonance
|X-Ray Diffraction (XRD)
Analysis of the structure and composition of crystalline and amorphous materials using X-ray diffraction measurements are performed at our center using a Bruker XRD device, model D8 Advance (powder analysis).
· Spectral analysis and consonance
HORIBA Jobin Yvon Raman spectroscopy is used to measure Raman spectra of microscopic samples or microscopic areas of larger objects.
· Micro Raman
· Spectral analysis and consonance
|Nuclear Magnetic Resonance Facility (NMR)
Bar Ilans nuclear magnetic resonance (NMR) unit is one of the largest in Israel and includes 6 Bruker spectrometer devices (frequencies of 200-700MHz for protons), with one of the instruments dedicated for solids, and the other with a cryo probe for work with proteins and samples at low concentrations. All devices are suitable for 2D experiments and are suitable for nuclides such as 13C, 15N, 31P, 25Mg, 27Al etc.
· Hour of instrument time
· Overnight run (e.g. 13C for a dilute sample)
· Spectral analysis and consultation
|Mass Spectrometry Unit (MS)
The unit provides mass spectra (MS) analysis for solutions, gasses and solids. This unit is equipped with GC-MS, HPLC-MS, MALDI and TGA-MS. A variety of quantitative and qualitative analyses (such as HRMS and MS/MS) is provided for both synthetic and natural substances. Professional consultation and interpretation will be given on demand.
· GC-EI/CI first injection, up to 6 peaks
· Additional injections
· GC working day (7 hours)
· TGA analysis
· TGA-MS analysis
· LC-MS first injection
· Additional injection
· LC-MS working day
· Spectral analysis and consultancy